Nitrofurantoin ELISA Kit
Katalog-Nummer OKAO00103
Size : 96Wells
Marke : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for Nitrofurantoin ELISA Kit (OKAO00103) |
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Predicted Species Reactivity | General | ||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Nitrofurantoin ELISA Kit (OKAO00103) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Nitrofurantoin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Nitrofurantoin antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample. | ||||||||||||||||||||||
ELISA Kit Range | 0.02-1.62 ppb | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay: CV<8% (n= 3 x 20) Mean Inter-Assay: CV<12% (n= 3 x 40) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | CAS: 67-20-9 PubChem: 6604200 | ||||||||||||||||||||||
:: | Molecular Formula: C8H6N4O5 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Tissue (Chicken, duck, porcine meat, beef and mutton, fish, shrimp), liver (Chicken, duck, porcine meat, beef and mutton), honey, milk, intestine. | ||||||||||||||||||||||
Sensitivity |
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Specificity |
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Assay Info | Assay Methodology: Competitive Inhibition ELISA |
Alias Symbols | AHD, Nitrofurantoin, 67-20-9, Macrodantin, 5-Nitrofurantoin, Furadantine, Furadonine |
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Protein Name | Nitrofurantoin |
Description of Target | Nitrofurantoin is a synthetic derivative of imidazolidinedione, Nitrofurantoin inhibits bacterial DNA, RNA, and cell wall protein synthesis. Activated by bacterial flavoproteins to intermediates that inactivate bacterial ribosomal proteins, Nitrofurantoin is used prophylactically as a urinary anti-infective agent against most gram-positive and gram-negative organisms and for long-term suppression of infections. |
Molecular Weight | 238.15704 g/mol |