Nitrofurantoin ELISA Kit

Katalog-Nummer OKAO00103

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Nitrofurantoin ELISA Kit (OKAO00103)
Product Info
Predicted Species ReactivityGeneral
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology Nitrofurantoin ELISA Kit (OKAO00103) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Nitrofurantoin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Nitrofurantoin antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
ELISA Kit Range0.02-1.62 ppb
ELISA Kit Recovery
SampleAmount
Tissue, Liver90% +/- 15%
Honey, milk80% +/- 15%
Intestine80% +/- 15%
ELISA Kit ReproducibilityMean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
ELISA Kit Component
ComponentAmount
Nitrofurantoin Microplate96 Wells (12 x 8 Well Strips)
Nitrofurantoin Standards6 x 1 mL
Nitrofurantoin Rabbit Detector Antibody1 x 7 mL
Anti-Rabbit / HRP Conjugate1 x 7 mL
Detection Reagent A1 x 7 mL
Detection Reagent B1 x 7 mL
Stop Solution1 x 7 mL
20X Wash Buffer2 x 20 mL
2X Sample Prep Buffer2 x 20 mL
2-Nitrobenzaldehyde (C7H5NO3)1 x 10 mL
Additional InformationCAS: 67-20-9
PubChem: 6604200
::Molecular Formula: C8H6N4O5
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue (Chicken, duck, porcine meat, beef and mutton, fish, shrimp), liver (Chicken, duck, porcine meat, beef and mutton), honey, milk, intestine.
Sensitivity
SampleAmount
Tissue0.1 ppb
Liver0.1 ppb
Honey, milk0.1 ppb
Intestine0.1 ppb
Specificity
SubstanceCross Reactivity Rate
AHD100%
SEM<0.1%
AOZ<0.1%
AMOZ<0.1%
Assay InfoAssay Methodology: Competitive Inhibition ELISA
Alias SymbolsAHD, Nitrofurantoin, 67-20-9, Macrodantin, 5-Nitrofurantoin, Furadantine, Furadonine
Protein NameNitrofurantoin
Description of TargetNitrofurantoin is a synthetic derivative of imidazolidinedione, Nitrofurantoin inhibits bacterial DNA, RNA, and cell wall protein synthesis. Activated by bacterial flavoproteins to intermediates that inactivate bacterial ribosomal proteins, Nitrofurantoin is used prophylactically as a urinary anti-infective agent against most gram-positive and gram-negative organisms and for long-term suppression of infections.
Molecular Weight238.15704 g/mol