Nicotinic Acid Adenine Dinucleotide Phosphate ELISA Kit
Katalog-Nummer OKCD02279
Size : 96Wells
Marke : Aviva Systems Biology
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| Datasheets/Manuals | Printable datasheet for Nicotinic Acid Adenine Dinucleotide Phosphate ELISA Kit (OKCD02279) |
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| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 2h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) and unlabeled Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) (Standards or samples) with the pre-coated antibody specific to Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) in the sample. | ||||||||||||||||||||||
| ELISA Kit Range | 123.5-10,000ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | CAS: 5502-96-5 PubChem: 123953 | ||||||||||||||||||||||
| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 45.8 ng/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Competitive Inhibition Immunoassay |
| Gene Symbol | NAADP |
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| Alias Symbols | NAADP; NAADP+; 1-[(2R,3R,4S,5R)-5-[[[[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxymethyl]-3,4-dihydroxyoxolan-2-yl]pyridin-1-ium-3-carboxylic acid; Nicotinic acid adenine dinuc |
| Protein Name | Nicotinic Acid Adenine Dinucleotide Phosphate |