Malachite green ELISA Kit

Katalog-Nummer OKAO00134

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Malachite green ELISA Kit (OKAO00134)
Product Info
Predicted Species ReactivityGeneral
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology Malachite green ELISA Kit (OKAO00134) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Malachite green has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Malachite green antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
ELISA Kit Range0.5-8 ppb
ELISA Kit Recovery
SampleAmount
Tissue85% +/- 20%
ELISA Kit ReproducibilityMean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
ELISA Kit Component
ComponentAmount
Malachite green Microplate96 Wells (12 x 8 Well Strips)
Malachite green Standards6 x 1 mL
Malachite green Rabbit Detector Antibody1 x 7 mL
Anti-Rabbit / HRP Conjugate1 x 0.7 mL
Detection Reagent A1 x 7 mL
Detection Reagent B1 x 7 mL
Stop Solution1 x 7 mL
20X Wash Buffer2 x 15 mL
Sample Prep Buffer5 x 20 mL
Additional InformationCAS: 569-64-2
PubChem: 11294
::Molecular Formula: C23H25ClN2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue (fish, shrimp)
Sensitivity
SampleAmount
Tissue0.2 ppb
Specificity
SubstanceCross Reactivity Rate
Malachite green100%
Leucomalachite green100%
Assay InfoAssay Methodology: Competitive Inhibition ELISA
Alias SymbolsMG, MALACHITE GREEN, Basic Green 4, Victoria Green B, Malachite green chloride, Aniline Green, Grenoble Green
Protein NameMalachite green
Description of TargetC.i. basic green 4 is a green crystals with metallic luster. Water solutions are blue-green. Used in dyeing silk, wool, biological staining, etc.
Molecular Weight364.911 g/mol