Lipoxin A4 ELISA Kit

Katalog-Nummer OKEH02592

Size : 96Wells

Marke : Aviva Systems Biology

Contact local distributor :


Telefonnummer : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02592
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Lipoxin A4 ELISA Kit (OKEH02592) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Lipoxin A4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Lipoxin A4 and incubated. The Lipoxin A4 found in the sample or standards competes with the biotinylated Lipoxin A4 for limited binding sites on the immobilized anti-Lipoxin A4 antibody. Excess unbound biotinylated Lipoxin A4 and sample or standard Lipoxin A4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Lipoxin A4 captured in the well and inversely proportional to the amount of Lipoxin A4 which was contained in the sample or standard.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit Recovery<= 6.2%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.1% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Lipoxin A4 Microplate96 Wells (12 x 8 Well strips)
Lipoxin A4 Lyophilized Standard2 x 2,000 pg
Sample Diluent1 x 20 mL
100X Lipoxin A4-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: LXA4
Target Name: Lipoxin A4
::Pubchem: 5280914
::Chemical formula: C20H32O5
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity9.6 pg/mL
SpecificityNatural and recombinant General Lipoxin A4
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsLXA4, Lipoxin A4, LXA4
Protein NameLipoxin A4
Description of TargetLipoxins are a series of anti-inflammatory mediators. Lipoxins are short lived endogenously produced nonclassic eicosanoids whose appearance in inflammation signals the resolution of inflammation. They are abbreviated as LX, an acronym for lipoxygenase (LO) interaction products. At present two lipoxins have been identified; lipoxin A4 (LXA4) and lipoxin B4 (LXB4).