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> Leukotriene A4 ELISA Kit

Leukotriene A4 ELISA Kit

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Katalog-Nummer OKCD02275

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Leukotriene A4 ELISA Kit (OKCD02275)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)81-88%93-101%79-104%95-102%
EDTA plasma(n=5)94-103%85-96%88-95%93-105%
heparin plasma(n=5)78-102%79-104%93-105%93-102%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Leukotriene A4 (LTA4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Leukotriene A4 (LTA4) and unlabeled Leukotriene A4 (LTA4) (Standards or samples) with the pre-coated antibody specific to Leukotriene A4 (LTA4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Leukotriene A4 (LTA4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Leukotriene A4 (LTA4) in the sample.
ELISA Kit Range123.5-10,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)88-9993
EDTA plasma(n=5)95-104101
heparin plasma(n=5)81-8885
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leukotriene A4 (LTA4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leukotriene A4 (LTA4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Leukotriene A4 Microplate96 Wells (12 x 8 Well Strips)
Leukotriene A4 Lyophilized Standard2
100X Leukotriene A4 Biotin Complex120 uL
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Additional InformationCAS: 72059-45-1
PubChem: 5280383
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sensitivity46.3 pg/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene SymbolLTA4
Alias SymbolsLeukotriene A4; LTA4; 5S,6S-epoxy-7E,9E,11Z,14Z-eicosatetraenoic acid
Protein NameLeukotriene A4
Description of Target(2S-(2 alpha,3 beta(1E,3E,5Z,8Z)))-3-(1,3,5,8-Tetradecatetraenyl)oxiranebutanoic acid. An unstable allylic epoxide, formed from the immediate precursor 5-HPETE via the stereospecific removal of a proton at C-10 and dehydration. Its biological actions are determined primarily by its metabolites, i.e., LEUKOTRIENE B4 and cysteinyl-leukotrienes. Alternatively, leukotriene A4 is converted into LEUKOTRIENE C4 by glutathione-S-transferase or into 5,6-di-HETE by the epoxide-hydrolase. (From Dictionary of Prostaglandins and Related Compounds, 1990)