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Lactic Acid ELISA Kit

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Katalog-Nummer OKCD02272

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Lactic Acid ELISA Kit (OKCD02272)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)79-92%98-105%80-101%79-92%
EDTA plasma(n=5)94-103%86-101%88-97%98-105%
heparin plasma(n=5)96-104%93-105%94-105%85-95%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Lactic Acid (LA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Lactic Acid (LA) and unlabeled Lactic Acid (LA) (Standards or samples) with the pre-coated antibody specific to Lactic Acid (LA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Lactic Acid (LA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Lactic Acid (LA) in the sample.
ELISA Kit Range7.41-600ug/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)89-10197
EDTA plasma(n=5)93-10297
heparin plasma(n=5)85-10189
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactic Acid (LA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactic Acid (LA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Lactic Acid Microplate96 Wells (12 x 8 Well Strips)
Lactic Acid Lyophilized Standard2
Lactic Acid Biotin Complex1
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Biotin Complex Reconstitution Buffer1 x 300 uL
Additional InformationMolecular Formula: C3H6O3 or CH3CHOHCOOH or HC3H5O3
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity2.92 ug/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene SymbolLA
Alias SymbolsLactic acid; 2-hydroxypropanoic acid; DL-Lactic acid; 2-hydroxypropionic acid
Protein NameLactic Acid
Description of TargetLactic acid is a colorless to yellow odorless syrupy liquid. Corrosive to metals and tissue. Used to make cultured dairy products, as a food preservative, and to make chemicals.