Kynurenic Acid ELISA Kit
Katalog-Nummer OKCD02271
Size : 96Wells
Marke : Aviva Systems Biology
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| Datasheets/Manuals | Printable datasheet for Kynurenic Acid ELISA Kit (OKCD02271) |
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| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 2h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Kynurenic Acid (KYNA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Kynurenic Acid (KYNA) and unlabeled Kynurenic Acid (KYNA) (Standards or samples) with the pre-coated antibody specific to Kynurenic Acid (KYNA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Kynurenic Acid (KYNA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Kynurenic Acid (KYNA) in the sample. | ||||||||||||||||||||||
| ELISA Kit Range | 2.47-200ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kynurenic Acid (KYNA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kynurenic Acid (KYNA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | CAS: 492-27-3 PubChem: 3845 | ||||||||||||||||||||||
| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 9.24 ng/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Competitive Inhibition Immunoassay |
| Gene Symbol | KYNA |
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| Alias Symbols | Kynurenic acid; 4-Hydroxyquinoline-2-carboxylic acid; 492-27-3; Quinurenic acid; Kynurenate |
| Protein Name | Kynurenic Acid |
| Description of Target | Kynurenic acid is a broad-spectrum excitatory amino acid antagonist used as a research tool. |