Homovanillic acid ELISA Kit

Katalog-Nummer OKEH02589

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Homovanillic acid ELISA Kit (OKEH02589) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Homovanillic acid antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Homovanillic acid and incubated. The Homovanillic acid found in the sample or standards competes with the biotinylated Homovanillic acid for limited binding sites on the immobilized anti-Homovanillic acid antibody. Excess unbound biotinylated Homovanillic acid and sample or standard Homovanillic acid is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Homovanillic acid captured in the well and inversely proportional to the amount of Homovanillic acid which was contained in the sample or standard.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Homovanillic acid Microplate96 Wells (12 x 8 Well strips)
Homovanillic acid Lyophilized Standard2 x 20 nmol
Sample Diluent1 x 20 mL
100X Homovanillic acid-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: HVA
Target Name: Homovanillic acid
::Pubchem: 1738
::Chemical formula: C9H10O4
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.29 ng/mL
SpecificityNatural and recombinant General Homovanillic acid
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsHVA, Homovanillic acid, HVA
Protein NameHomovanillic acid
Description of TargetHomovanillic acid (HOC6H3(OCH3)CH2COOH; synonyms: 3-Methoxy-4-hydroxyphenyl acetic acid; HVA; 4-Hydroxy-3-methoxy-benzeneacetic acid; 4-Hydroxy-3-methoxyphenylacetic acid) is a major catecholamine metabolite. It is used as a reagent to detect oxidative enzymes, and is associated with dopamine levels in the brain. In psychiatry and neuroscience, brain and cerebrospinal fluid levels of HVA are measured as a marker of metabolic stress caused by 2-deoxy-D-glucose. HVA presence supports a diagnosis of neuroblastoma and malignant pheochromocytoma. Fasting plasma levels of HVA are known to be higher in females than in males. This does not seem to be influenced by adult hormonal changes, as the pattern is retained in the elderly and post-menopausal as well as transsexuals according to their genetic sex, both before and during cross-sex hormone administration. Differences in HVA have also been correlated to tobacco usage, with smokers showing significantly lower amounts of plasma HVA.