Fumonisin B1 ELISA Kit
Katalog-Nummer OKAO00145
Size : 96Wells
Marke : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for Fumonisin B1 ELISA Kit (OKAO00145) |
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Predicted Species Reactivity | General | ||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Fumonisin B1 ELISA Kit (OKAO00145) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Fumonisin B1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Fumonisin B1 antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample. | ||||||||||||||||||||
ELISA Kit Range | 0.5-40.5 ppb | ||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay: CV<8% (n= 3 x 20) Mean Inter-Assay: CV<12% (n= 3 x 40) | ||||||||||||||||||||
ELISA Kit Component |
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Additional Information | CAS: 116355-83-0 PubChem: 3431 | ||||||||||||||||||||
:: | Molecular Formula: C34H59NO15 | ||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||
Sample Type | Corn,feed and cooking oil | ||||||||||||||||||||
Sensitivity |
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Specificity |
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Assay Info | Assay Methodology: Competitive Inhibition ELISA |
Alias Symbols | FB1, Fumonisin b1, Macrofusine, Fumonisin B1, Fusarium moniliforme, NSC629151, 116355-83-0, FB1 |
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Protein Name | Fumonisin B1 |
Description of Target | Fumonisin B1 is the most prevalent member of a family of toxins, known as fumonisins, produced by several species of Fusarium molds, such as Fusarium verticillioides,[1] which occur mainly in maize (corn), wheat and other cereals. Fumonisin B1 contamination of maize has been reported worldwide at mg/kg levels. Human exposure occurs at levels of micrograms to milligrams per day and is greatest in regions where maize products are the dietary staple. |
Molecular Weight | 721.82996 g/mol |