Fumonisin B1 ELISA Kit

Katalog-Nummer OKAO00145

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Fumonisin B1 ELISA Kit (OKAO00145)
Product Info
Predicted Species ReactivityGeneral
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology Fumonisin B1 ELISA Kit (OKAO00145) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Fumonisin B1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Fumonisin B1 antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
ELISA Kit Range0.5-40.5 ppb
ELISA Kit Recovery
SampleAmount
Corn100% +/- 15%
Feed95% +/- 15%
Cooking oil85% +/- 15%
ELISA Kit ReproducibilityMean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
ELISA Kit Component
ComponentAmount
Fumonisin B1 Microplate96 Wells (12 x 8 Well Strips)
Fumonisin B1 Standards6 x 1 mL
Fumonisin B1 Rabbit Detector Antibody1 x 6 mL
Anti-Rabbit / HRP Conjugate1 x 6 mL
Detection Reagent A1 x 6 mL
Detection Reagent B1 x 6 mL
Stop Solution1 x 6 mL
20X Wash Buffer1 x 20 mL
10X Sample Prep Buffer2 x 20 mL
Additional InformationCAS: 116355-83-0
PubChem: 3431
::Molecular Formula: C34H59NO15
Reconstitution and StorageStore as indicated in product manual.
Sample TypeCorn,feed and cooking oil
Sensitivity
SampleAmount
Corn, Feed50 ppb
Cooking oil10 ppb
Specificity
SubstanceCross Reactivity Rate
Fumonisin B1100%
Assay InfoAssay Methodology: Competitive Inhibition ELISA
Alias SymbolsFB1, Fumonisin b1, Macrofusine, Fumonisin B1, Fusarium moniliforme, NSC629151, 116355-83-0, FB1
Protein NameFumonisin B1
Description of TargetFumonisin B1 is the most prevalent member of a family of toxins, known as fumonisins, produced by several species of Fusarium molds, such as Fusarium verticillioides,[1] which occur mainly in maize (corn), wheat and other cereals. Fumonisin B1 contamination of maize has been reported worldwide at mg/kg levels. Human exposure occurs at levels of micrograms to milligrams per day and is greatest in regions where maize products are the dietary staple.
Molecular Weight721.82996 g/mol