Dopamine ELISA Kit
Katalog-Nummer OKEH02560
Size : 96Wells
Marke : Aviva Systems Biology
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Telefonnummer : +1 850 650 7790
| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Dopamine ELISA Kit (OKEH02560) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Dopamine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Dopamine and incubated. The Dopamine found in the sample or standards competes with the biotinylated Dopamine for limited binding sites on the immobilized anti-Dopamine antibody. Excess unbound biotinylated Dopamine and sample or standard Dopamine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Dopamine captured in the well and inversely proportional to the amount of Dopamine which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 1.56-100ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 6.1% (n = 20) Mean Inter-assay CV%: < 10.2% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: DA Target Name: Dopamine | ||||||||||||||||||||||
| :: | Pubchem: 681 | ||||||||||||||||||||||
| :: | Chemical formula: C8H11NO2 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 4.1 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General Dopamine | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Alias Symbols | DA, Dopamine, DA |
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| Protein Name | Dopamine |
| Description of Target | In the brain, dopamine functions as a neurotransmitter—a chemical released by neurons (nerve cells) to send signals to other nerve cells. The brain includes several distinct dopamine pathways, one of which plays a major role in reward-motivated behavior. Most types of rewards increase the level of dopamine in the brain, and many addictive drugs increase dopamine neuronal activity. Other brain dopamine pathways are involved in motor control and in controlling the release of various hormones. These pathways and cell groups form a dopamine system which is neuromodulatory. Outside the central nervous system, dopamine functions primarily as a local chemical messenger. In blood vessels, it inhibits norepinephrine release and acts as a vasodilator (at normal concentrations); in the kidneys, it increases sodium excretion and urine output; in the pancreas, it reduces insulin production; in the digestive system, it reduces gastrointestinal motility and protects intestinal mucosa; and in the immune system, it reduces the activity of lymphocytes. With the exception of the blood vessels, dopamine in each of these peripheral systems is synthesized locally and exerts its effects near the cells that release it. |