Deoxypyridinoline ELISA Kit

Katalog-Nummer OKEH02563

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02563
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Deoxypyridinoline ELISA Kit (OKEH02563) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Deoxypyridinoline antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Deoxypyridinoline and incubated. The Deoxypyridinoline found in the sample or standards competes with the biotinylated Deoxypyridinoline for limited binding sites on the immobilized anti-Deoxypyridinoline antibody. Excess unbound biotinylated Deoxypyridinoline and sample or standard Deoxypyridinoline is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Deoxypyridinoline captured in the well and inversely proportional to the amount of Deoxypyridinoline which was contained in the sample or standard.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 114%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 10.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Deoxypyridinoline Microplate96 Wells (12 x 8 Well strips)
Deoxypyridinoline Lyophilized Standard2 x 100 ng
Sample Diluent1 x 20 mL
100X Deoxypyridinoline-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: DPD
Target Name: Deoxypyridinoline
::Pubchem: 105071
::Chemical formula: C18H28N4O7
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.47 ng/mL
SpecificityNatural and recombinant General Deoxypyridinoline
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsDPD, Deoxypyridinoline, DPD
Protein NameDeoxypyridinoline
Description of TargetDeoxypyridinoline, also called D-Pyrilinks, Pyrilinks-D, or deoxyPYD, is one of two pyridinium cross-links that provide structural stiffness to type I collagen found in bones. It is excreted unmetabolized in urine and is a specific marker of bone resorption and osteoclastic activity. It is measured in urine tests and is used along with other bone markers such as alkaline phosphatase, osteocalcin, and N-terminal telopeptide to diagnose bone diseases such as postmenopausal osteoporosis, bone metastasis, and Paget's disease, furthermore, it has been useful in monitoring treatments that conatin bone-active agents such as estrogens and bisphosphonates.

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MBS7230400-96
 96-Strip-Wells