Deoxypyridinoline ELISA Kit
Katalog-Nummer OKEH02563
Size : 96Wells
Marke : Aviva Systems Biology
Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Deoxypyridinoline ELISA Kit (OKEH02563) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Deoxypyridinoline antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Deoxypyridinoline and incubated. The Deoxypyridinoline found in the sample or standards competes with the biotinylated Deoxypyridinoline for limited binding sites on the immobilized anti-Deoxypyridinoline antibody. Excess unbound biotinylated Deoxypyridinoline and sample or standard Deoxypyridinoline is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Deoxypyridinoline captured in the well and inversely proportional to the amount of Deoxypyridinoline which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 1.56-100ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 114% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 6.1% (n = 20) Mean Inter-assay CV%: < 10.6% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Target Abbr: DPD Target Name: Deoxypyridinoline | ||||||||||||||||||||||
:: | Pubchem: 105071 | ||||||||||||||||||||||
:: | Chemical formula: C18H28N4O7 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.47 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant General Deoxypyridinoline | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Alias Symbols | DPD, Deoxypyridinoline, DPD |
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Protein Name | Deoxypyridinoline |
Description of Target | Deoxypyridinoline, also called D-Pyrilinks, Pyrilinks-D, or deoxyPYD, is one of two pyridinium cross-links that provide structural stiffness to type I collagen found in bones. It is excreted unmetabolized in urine and is a specific marker of bone resorption and osteoclastic activity. It is measured in urine tests and is used along with other bone markers such as alkaline phosphatase, osteocalcin, and N-terminal telopeptide to diagnose bone diseases such as postmenopausal osteoporosis, bone metastasis, and Paget's disease, furthermore, it has been useful in monitoring treatments that conatin bone-active agents such as estrogens and bisphosphonates. |