cyclicAMP ELISA Kit

Katalog-Nummer OKEH02525

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology cyclicAMP ELISA Kit (OKEH02525) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-cyclicAMP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated cyclicAMP and incubated. The cyclicAMP found in the sample or standards competes with the biotinylated cyclicAMP for limited binding sites on the immobilized anti- cyclicAMP antibody. Excess unbound biotinylated cyclicAMP and sample or standard cyclicAMP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated cyclicAMP captured in the well and inversely proportional to the amount of cyclicAMP which was contained in the sample or standard.
ELISA Kit Range0.312-20nmol/L
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.1% (n = 20)
Mean Inter-assay CV%: < 7.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Adenosine triphosphate Microplate96 Wells (12 x 8 Well strips)
Adenosine triphosphate Lyophilized Standard2 x 20 nmol
Sample Diluent1 x 20 mL
100X Adenosine triphosphate-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: cyclicAMP
Target Name: Cyclic adenosine monophosphate
::Pubchem: 6076
::Chemical formula: C10H12N5O6P
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.096nmol/L
SpecificityNatural and recombinant General Cyclic adenosine monophosphate
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolscyclicAMP, Cyclic adenosine monophosphate, cyclicAMP, cAMP, cyclic AMP
Protein NamecyclicAMP
Description of TargetcAMP is a second messenger, used for intracellular signal transduction, such as transferring into cells the effects of hormones like glucagon and adrenaline, which cannot pass through the cell membrane. It is involved in the activation of protein kinases and regulates the effects of adrenaline and glucagon. cAMP also binds to and regulates the function of ion channels such as the HCN channels and a few other cyclic nucleotide-binding proteins such as Epac1. cAMP is synthesised from ATP by adenylyl cyclase located on the inner side of the plasma membrane. Adenylyl cyclase is activated by a range of signaling molecules through the activation of adenylyl cyclase stimulatory G (Gs)-protein-coupled receptors and inhibited by agonists of adenylyl cyclase inhibitory G (Gi)-protein-coupled receptors. Liver adenylyl cyclase responds more strongly to glucagon, and muscle adenylyl cyclase responds more strongly to adrenaline. cAMP decomposition into AMP is catalyzed by the enzyme phosphodiesterase.

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