Cholylglycine ELISA Kit

Katalog-Nummer OKEH02529

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Cholyglycine ELISA Kit (OKEH02529) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Cholyglycine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Cholyglycine and incubated. The Cholyglycine found in the sample or standards competes with the biotinylated Cholyglycine for limited binding sites on the immobilized anti-Cholyglycine antibody. Excess unbound biotinylated Cholyglycine and sample or standard Cholyglycine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Cholyglycine captured in the well and inversely proportional to the amount of Cholyglycine which was contained in the sample or standard.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.4% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Cholyglycine Microplate96 Wells (12 x 8 Well strips)
Cholyglycine Lyophilized Standard2 x 100 ng
Sample Diluent1 x 20 mL
100X Cholyglycine-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: cholyglycine
Target Name: cholyglycine
::Pubchem: 10140
::Chemical formula: C26H43NO6
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.5 ng/mL
SpecificityNatural and recombinant General cholyglycine
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsGlycocoholic acid
Protein NameCholylglycine
Description of Targeterum Cholygllycine (Cholygllycine, CG) is a combination of secondary bile acids with glycine conjugated bile acids, in liver cells, cholesterol, after its complex enzymatic reaction, turn into the primary bile acid. Cholic acid (CA) and chenodeoxycholic acid (the CD-CA). Bile acid steroid nucleus three hydroxyl groups (C3, C7, C12), the side chain hydroxyl at the end of the peptide bond and glycine combined with a molecular weight of 462u. CG normal metabolic pathways of the intestine - liver circulation, CG synthesized by hepatocytes, bile canaliculi, bile duct discharged into the gall bladder, along with the bile into the duodenum to help digestion of food. 95% of bile acid reabsorption in the distal ileum, come back to the liver via the portal vein, and uptake by the liver cells to re-use. Exist in the form of protein binding in serum, the total amount of overflow into the systemic circulation is less than 1%. Under normal circumstances, bile acid content in the peripheral blood, leaving little room for normal adults, whether fasting or postprandial serum CG concentration stabilized at a low level. When liver cells are damaged, the liver cell uptake CG capacity decreased, resulting in increased CG content in the blood; cholestasis, liver excretion of bile acids that barriers to reflux of blood circulation increased CG content, but also to the blood CG levels increased. Therefore, the determination of serum glycocholic acid (SCG) by ELISA method is a sensitive indicator to evaluate the function of liver cells and their hepatobiliary material circulation. Normal serum Cholygllycine: 1.3 ± 0.8mg / L, range 0.4 to 2.98mg / L, and the low limit of the hepatitis diagnosis <3.18mg / L