Angiotensin II ELISA Kit

Katalog-Nummer OKEH02526

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02526
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Angiotensin II ELISA Kit (OKEH02526) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Angiotensin II antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Angiotensin II and incubated. The Angiotensin II found in the sample or standards competes with the biotinylated Angiotensin II for limited binding sites on the immobilized anti-Angiotensin II antibody. Excess unbound biotinylated Angiotensin II and sample or standard Angiotensin II is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Angiotensin II captured in the well and inversely proportional to the amount of Angiotensin II which was contained in the sample or standard.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 91%
ELISA Kit ReproducibilityMean Intra-Assay Precision: %CV < 5.5%, n=20
Mean Inter-Assay Precision: %CV < 8.1%, n=20%
ELISA Kit Component
ComponentAmount
Anti-Angiotensin II Microplate96 Wells (12 x 8 Well strips)
Angiotensin II Lyophilized Standard2 x 2,000 pg
Sample Diluent1 x 20 mL
100X Angiotensin II-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: Ang-II
Target Name: Angiotensin II
::Pubchem: 172198
::Chemical formula: C50H71N13O12
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity10.2 pg/mL
SpecificityNatural and recombinant General Angiotensin II
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsAng-II, Angiotensin II, Ang-II
Description of TargetAngiotensin II is an octapeptide that is a potent but labile vasoconstrictor. It is produced from angiotensin I after the removal of two amino acids at the C-terminal by Angiotensin converting enzyme. The amino acid in position 5 varies in different species. To block vasoconstriction and hypertension effect of angiotensin II, patients are often treated with ace inhibitors or with Angiotensin II Type I receptor blockers.

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