Adenosine triphosphate ELISA Kit

Katalog-Nummer OKEH02624

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology General Adenosine triphosphate ELISA Kit (OKEH02624) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Adenosine triphosphate antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Adenosine triphosphate and incubated. The Adenosine triphosphate found in the sample or standards competes with the biotinylated Adenosine triphosphate for limited binding sites on the immobilized anti-Adenosine triphosphate antibody. Excess unbound biotinylated Adenosine triphosphate and sample or standard Adenosine triphosphate is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Adenosine triphosphate captured in the well and inversely proportional to the amount of Adenosine triphosphate which was contained in the sample or standard.
ELISA Kit Range1.56-100ng/ml
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 84%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Adenosine triphosphate Microplate96 Wells (12 x 8 Well strips)
Adenosine triphosphate Lyophilized Standard2 x 200 ng
Sample Diluent1 x 20 mL
100X Adenosine triphosphate-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: ATP
Target Name: Adenosine triphosphate
::Pubchem: 5957
::Chemical formula: C10H16N5O13P3
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.35 ng/mL
SpecificityNatural and recombinant General Adenosine triphosphate
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsATP, Adenosine triphosphate
Protein NameAdenosine triphosphate
Description of TargetAdenosine-5'-triphosphate (ATP) is a multifunctional nucleoside triphosphate used in cells as a coenzyme. It is often called the "molecular unit of currency" of intracellular energy transfer. ATP transports chemical energy within cells for metabolism. It is one of the end products of photophosphorylation and cellular respiration and used by enzymes and structural proteins in many cellular processes, including biosynthetic reactions, motility, and cell division. One molecule of ATP contains three phosphate groups, and it is produced by ATP synthase from inorganic phosphate and adenosine diphosphate (ADP) or adenosine monophosphate (AMP). The three main ways of ATP synthesis is substrate level phosphorylation and oxidative phosphorylation in cellular respiration, and photophosphorylation in photosynthesis.

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