Acetylcarnitine ELISA Kit
Katalog-Nummer OKCD02252
Size : 96Wells
Marke : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for Acetylcarnitine ELISA Kit (OKCD02252) |
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Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 2h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Acetylcarnitine (ALCAR) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Acetylcarnitine (ALCAR) and unlabeled Acetylcarnitine (ALCAR) (Standards or samples) with the pre-coated antibody specific to Acetylcarnitine (ALCAR). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Acetylcarnitine (ALCAR) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Acetylcarnitine (ALCAR) in the sample. | ||||||||||||||||||||||
ELISA Kit Range | 61.7-5,000ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acetylcarnitine (ALCAR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acetylcarnitine (ALCAR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 22.9 ng/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Competitive Inhibition Immunoassay |
Gene Symbol | ALCAR |
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Alias Symbols | ALCAR; Acetyl-L-carnitine; Acetylcarnitine; O-Acetyl-L-carnitine; L-ACETYLCARNITINE; Levocarnitine acetyl; L-O-Acetylcarnitine |
Protein Name | Acetylcarnitine |
Description of Target | L-Acetylcarnitine (ALCAR or ALC) is an acetic acid ester of carnitine that facilitates movement of acetyl-CoA into the matrices of mammalian mitochondria during the oxidation of fatty acids. In addition to his metabolic role, acetyl-L-carnitine posses unique neuroprotective, neuromodulatory, and neurotrophic properties this may play an important role in counteracting various disease processes. |