Acetylcarnitine ELISA Kit

Katalog-Nummer OKCD02252

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Acetylcarnitine ELISA Kit (OKCD02252)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)98-105%95-103%98-105%78-101%
EDTA plasma(n=5)80-93%86-101%95-102%86-104%
heparin plasma(n=5)89-103%96-105%84-104%98-105%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Acetylcarnitine (ALCAR) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Acetylcarnitine (ALCAR) and unlabeled Acetylcarnitine (ALCAR) (Standards or samples) with the pre-coated antibody specific to Acetylcarnitine (ALCAR). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Acetylcarnitine (ALCAR) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Acetylcarnitine (ALCAR) in the sample.
ELISA Kit Range61.7-5,000ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)80-9582
EDTA plasma(n=5)90-9894
heparin plasma(n=5)80-10299
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acetylcarnitine (ALCAR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acetylcarnitine (ALCAR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Acetylcarnitine Microplate96 Wells (12 x 8 Well Strips)
Acetylcarnitine Lyophilized Standard2
100X Acetylcarnitine Biotin Complex120 uL
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 22.9 ng/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene SymbolALCAR
Alias SymbolsALCAR; Acetyl-L-carnitine; Acetylcarnitine; O-Acetyl-L-carnitine; L-ACETYLCARNITINE; Levocarnitine acetyl; L-O-Acetylcarnitine
Protein NameAcetylcarnitine
Description of TargetL-Acetylcarnitine (ALCAR or ALC) is an acetic acid ester of carnitine that facilitates movement of acetyl-CoA into the matrices of mammalian mitochondria during the oxidation of fatty acids. In addition to his metabolic role, acetyl-L-carnitine posses unique neuroprotective, neuromodulatory, and neurotrophic properties this may play an important role in counteracting various disease processes.