5-Hydroxytryptophan ELISA Kit

Katalog-Nummer OKCD02250

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for 5-Hydroxytryptophan ELISA Kit (OKCD02250)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)97-104%98-105%95-102%86-103%
EDTA plasma(n=5)87-101%86-101%99-105%78-96%
heparin plasma(n=5)80-90%80-96%96-103%91-99%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 5-Hydroxytryptophan (5-HTP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 5-Hydroxytryptophan (5-HTP) and unlabeled 5-Hydroxytryptophan (5-HTP) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxytryptophan (5-HTP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Hydroxytryptophan (5-HTP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 5-Hydroxytryptophan (5-HTP) in the sample.
ELISA Kit Range4.93-400ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)94-10598
EDTA plasma(n=5)99-105102
heparin plasma(n=5)90-9794
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Hydroxytryptophan (5-HTP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Hydroxytryptophan (5-HTP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-5-Hydroxytryptophan Microplate96 Wells (12 x 8 Well Strips)
5-Hydroxytryptophan Lyophilized Standard2
100X 5-Hydroxytryptophan Biotin Complex120 uL
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Additional InformationCAS: 4350-09-8
PubChem: 439280
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity1.93 ng/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene Symbol5-HTP
Alias Symbols5-hydroxy-L-tryptophan; L-5-Hydroxytryptophan; Oxitriptan; 4350-09-8; 5-hydroxytryptophan
Protein Name5-Hydroxytryptophan
Description of Target5-Hydroxytryptophan is an aromatic amino acid with antidepressant activity. In vivo, oxitriptan (or 5-hydroxytryptophan) is converted into 5-hydroxytryptamine (5-HT or serotonin) as well as other neurotransmitters. Oxitriptan may exert its antidepressant activity via conversion to serotonin or directly by binding to serotonin (5-HT) receptors within the central nervous system (CNS). Endogenous oxitriptan is produced from the essential amino acid L-tryptophan. The exogenous therapeutic form is isolated from the seeds of the African plant Griffonia simplicifolia.