1-Desamino 8D Arginine Vasopressin ELISA Kit
Katalog-Nummer OKCD02265
Size : 96Wells
Marke : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for 1-Desamino 8D Arginine Vasopressin ELISA Kit (OKCD02265) |
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Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 2h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 1-Desamino 8D Arginine Vasopressin (DDAVP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 1-Desamino 8D Arginine Vasopressin (DDAVP) and unlabeled 1-Desamino 8D Arginine Vasopressin (DDAVP) (Standards or samples) with the pre-coated antibody specific to 1-Desamino 8D Arginine Vasopressin (DDAVP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 1-Desamino 8D Arginine Vasopressin (DDAVP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 1-Desamino 8D Arginine Vasopressin (DDAVP) in the sample. | ||||||||||||||||||||||
ELISA Kit Range | 246.9-20,000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 1-Desamino 8D Arginine Vasopressin (DDAVP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 1-Desamino 8D Arginine Vasopressin (DDAVP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | CAS: 16679-58-6 PubChem: 5311065 | ||||||||||||||||||||||
Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids. | ||||||||||||||||||||||
Sensitivity | 39.5 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Competitive Inhibition Immunoassay |
Gene Symbol | DDAVP |
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Alias Symbols | DESMOPRESSIN; 16679-58-6; DDAVP; UNII-ENR1LLB0FP |
Protein Name | 1-Desamino 8D Arginine Vasopressin |
Description of Target | Desmopressin is a Factor VIII Activator and Vasopressin Analog. The physiologic effect of desmopressin is by means of Increased Coagulation Factor VIII Activity and Increased Coagulation Factor VIII Concentration. The chemical classification of desmopressin is Vasopressins. |