1,5-Anhydroglucitol ELISA Kit

Katalog-Nummer OKCD02247

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)89-98%78-97%79-97%96-103%
EDTA plasma(n=5)88-98%86-93%78-101%93-105%
heparin plasma(n=5)99-105%86-104%78-94%91-99%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 1,5-Anhydroglucitol (1,5-AG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 1,5-Anhydroglucitol (1,5-AG) and unlabeled 1,5-Anhydroglucitol (1,5-AG) (Standards or samples) with the pre-coated antibody specific to 1,5-Anhydroglucitol (1,5-AG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 1,5-Anhydroglucitol (1,5-AG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 1,5-Anhydroglucitol (1,5-AG) in the sample.
ELISA Kit Range1.9-150ug/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)92-10597
EDTA plasma(n=5)94-10197
heparin plasma(n=5)95-10299
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 1,5-Anhydroglucitol (1,5-AG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 1,5-Anhydroglucitol (1,5-AG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-1,5-Anhydroglucitol Microplate96 Wells (12 x 8 Well Strips)
1,5-Anhydroglucitol Lyophilized Standard2
1,5-Anhydroglucitol Biotin Complex1
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Biotin Complex Reconstitution Buffer1 x 300 uL
Additional InformationPubChem: 64960
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 0.7 ug/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene Symbol1,5-AG
Alias Symbols1 1,5-Anhydro-D-glucitol; 1,5-Anhydroglucitol; 154-58-5; 1,5-ANHYDROSORBITOL
Protein Name1,5-Anhydroglucitol
Description of Target1, 5-Anhydrosorbitol or 1, 5-anhydroglucitol (1, 5-AG) is a validated marker of short-term glycemic control. This substance is derived mainly from food, is well absorbed in the intestine, and is distributed to all organs and tissues. It is metabolically stable, being excreted in the urine when its level exceeds the renal threshold. It is reabsorbed in the renal tubules and is competitively inhibited by glucosuria, which leads to a reduction in its level in serum. The correlation between this reduction and the amount of glucose present in urine is so close that 1, 5 AG can be used as a sensitive, day-to-day, real-time marker of glycemic control. It provides useful information on current glycemic control and is superior to both hemoglobin A1C and fructosamine in detecting near-normoglycemia. 1, 5-AG in human plasma has been proposed for several years as a short-term, retrospective marker of glycemic control and seems to be the most suitable parameter for monitoring glucose excursions. The decrease in serum 1, 5-AG is very sensitive to urinary glucose excretion. It is a metabolically inert polyol that competes with glucose for reabsorption in the kidneys. Otherwise stable levels of 1, 5-AG are rapidly depleted as blood glucose levels exceed the renal threshold for glucosuria. 1, 5-AG is also more tightly associated with glucose fluctuations and postprandial glucose.