1,5-Anhydroglucitol ELISA Kit
Katalog-Nummer OKCD02247
Size : 96Wells
Marke : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for 1,5-Anhydroglucitol ELISA Kit (OKCD02247) |
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COA Datasheet | Click Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
Predicted Species Reactivity | All Species | ||||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||||
ELISA Kit Duration | 2h | ||||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 1,5-Anhydroglucitol (1,5-AG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 1,5-Anhydroglucitol (1,5-AG) and unlabeled 1,5-Anhydroglucitol (1,5-AG) (Standards or samples) with the pre-coated antibody specific to 1,5-Anhydroglucitol (1,5-AG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 1,5-Anhydroglucitol (1,5-AG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 1,5-Anhydroglucitol (1,5-AG) in the sample. | ||||||||||||||||||||||||
ELISA Kit Range | 1.9-150ug/mL | ||||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 1,5-Anhydroglucitol (1,5-AG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 1,5-Anhydroglucitol (1,5-AG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | PubChem: 64960 | ||||||||||||||||||||||||
Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids. | ||||||||||||||||||||||||
Sensitivity | < 0.7 ug/mL | ||||||||||||||||||||||||
Assay Info | Assay Methodology: Competitive Inhibition Immunoassay |
Gene Symbol | 1,5-AG |
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Alias Symbols | 1 1,5-Anhydro-D-glucitol; 1,5-Anhydroglucitol; 154-58-5; 1,5-ANHYDROSORBITOL |
Protein Name | 1,5-Anhydroglucitol |
Description of Target | 1, 5-Anhydrosorbitol or 1, 5-anhydroglucitol (1, 5-AG) is a validated marker of short-term glycemic control. This substance is derived mainly from food, is well absorbed in the intestine, and is distributed to all organs and tissues. It is metabolically stable, being excreted in the urine when its level exceeds the renal threshold. It is reabsorbed in the renal tubules and is competitively inhibited by glucosuria, which leads to a reduction in its level in serum. The correlation between this reduction and the amount of glucose present in urine is so close that 1, 5 AG can be used as a sensitive, day-to-day, real-time marker of glycemic control. It provides useful information on current glycemic control and is superior to both hemoglobin A1C and fructosamine in detecting near-normoglycemia. 1, 5-AG in human plasma has been proposed for several years as a short-term, retrospective marker of glycemic control and seems to be the most suitable parameter for monitoring glucose excursions. The decrease in serum 1, 5-AG is very sensitive to urinary glucose excretion. It is a metabolically inert polyol that competes with glucose for reabsorption in the kidneys. Otherwise stable levels of 1, 5-AG are rapidly depleted as blood glucose levels exceed the renal threshold for glucosuria. 1, 5-AG is also more tightly associated with glucose fluctuations and postprandial glucose. |