MMP7 ELISA Kit (Cattle)

Katalog-Nummer OKCD04423

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for MMP7 ELISA Kit (Cattle) (OKCD04423)
Product Info
Predicted Species ReactivityBos taurus|Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)95-105%85-104%85-92%83-93%
Heparin Plasma (n=5)92-101%79-95%86-96%80-89%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 7 (MMP7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 7 (MMP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 7 (MMP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Matrix Metalloproteinase 7 (MMP7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)85-10491
Heparin Plasma (n=5)88-9794
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 7 (MMP7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 7 (MMP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Mmp7 Microplate96 Wells (12 x 8 Well strips)
Mmp7 Lyophilized Standard2
100X Mmp7 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity6.2 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMMP7
Gene Full Namematrix metallopeptidase 7
Alias Symbolsmatrilysin;matrix metallopeptidase 7 (matrilysin, uterine);matrix metalloproteinase 7 (matrilysin, uterine).
NCBI Gene Id286794
Protein NameMatrix metallopeptidase 7 (Matrilysin, uterine)
Description of TargetMatrix metallopeptidase 7 (Matrilysin, uterine)
Uniprot IDQ148N3
Protein Accession #NP_001068598.1
Nucleotide Accession #NM_001075130.1