Tri-iodothyronine ELISA Kit (Fish)

Katalog-Nummer OKCA00348

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKCA00348
Product Info
Predicted Species ReactivityFish
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration< 4 Hours
ELISA Kit PrincipleAviva Systems Biology Tri-iodothyronine ELISA Kit (Fish) (OKCA00348) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Fish Tri-iodothyronine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tri-iodothyronine and incubated. The Tri-iodothyronine found in the sample or standards competes with the biotinylated Tri-iodothyronine for limited binding sites on the immobilized anti-Fish Tri-iodothyronine antibody. Excess unbound biotinylated Tri-iodothyronine and sample or standard Tri-iodothyronine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tri-iodothyronine captured in the well and inversely proportional to the amount of Tri-iodothyronine which was contained in the sample or standard.
ELISA Kit Range0.5 ng/mL - 8 ng/mL
ELISA Kit Reproducibility
Mean Intra-Assay Precision %CVMean Inter-Assay Precision %CV
Sample123123
n202020202020

Mean (ng/mL

1.9871.9842.0011.9991.982.003
SD0.0170.0180.0230.0340.0310.029
CV%2.0132.1142.6413.9243.5263.376
ELISA Kit Component
ComponentAmount
Anti-Tri-iodothyronine Microplate96 Well (12 x 8 Well Strips)
Tri-iodothyronine Standard5 x 1 mL
Tri-iodothyronine-Biotin Complex1 x 6 mL
Avidin-HRP Conjugate1 x 6 mL
20X Wash Buffer1 x 15 mL
Substrate A1 x 7 mL
Substrate B1 x 7 mL
Stop Solution1 x 7 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates
Sensitivity0.25 ng/ml
SpecificityThis assay has high sensitivity and excellent specificity for detection of fish T3.
No significant cross-reactivity or interference between fish T3 and analogues
was observed.
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Description
Gene SymbolTri-iodothyronine
Alias Symbolstriiodothyronine, T3, 3,3',5-triiodo-L-thyronine
Protein NameTri-iodothyronine
Description of TargetThe L-triiodothyronine (T3, liothyronine) thyroid hormone is normally synthesized and secreted by the thyroid gland in much smaller quantities than L-tetraiodothyronine (T4, levothyroxine, L-thyroxine). Most T3 is derived from peripheral monodeiodination of T4 at the 5 position of the outer ring of the iodothyronine nucleus. The hormone finally delivered and used by the tissues is mainly T3.