LDH-A ELISA Kit (Goat)

Katalog-Nummer OKWB00144

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for LDH-A ELISA Kit (Goat) (OKWB00144)
Product Info
Predicted Species ReactivityCapra hircus|Goat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)86-104%85-99%87-97%86-100%
EDTA Plasma (n=5)83-98%88-100%86-95%86-95%
Heparin Plasma (n=5)81-96%83-96%86-98%85-97%
ELISA Kit PrincipleAviva Systems Biology Ldh-a ELISA Kit (Goat) (OKWB00144) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ldh-a has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ldh-a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ldh-a captured in the well.
ELISA Kit Range3.125-200mIU/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)87-9896
EDTA Plasma (n=5)94-10498
Heparin Plasma (n=5)85-10493
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
Ldh-a Microplate96 Wells (12 x 8 Well strips)
Ldh-a Lyophilized Standard2 x 200 U
100X Ldh-a Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity1.875 mIU/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolLDH-A
Gene Full Namelactate dehydrogenase
Alias Symbolslactate dehydrogenase;LDHA.
NCBI Gene Id100860870
Protein NameL-lactate dehydrogenase
Uniprot IDD1MGL8
Protein Accession #NP_001272647
Nucleotide Accession #NM_001285718