Goat IgG ELISA Kit (Goat)

Katalog-Nummer OKCA00272

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityGoat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:2,00085%80-94%
Serum (n=4)1:4,00088%83-99%
Serum (n=4)1:8,00092%86-104%
Serum (n=4)1:16,00096%89-108%
ELISA Kit PrincipleAviva Systems Biology Goat IgG ELISA Kit (Goat) (OKCA00272) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Goat IgG and blocked. Standards or samples are added to the plate wells along with a horseradish peroxidase (HRP) conjugated antibody specific to Goat IgG and incubated. Goat IgG immobilized in the wells competes with the liquid phase Goat IgG contained in the samples or standards for binding with the HRP-Conjugated Goat IgG Antibody. Wells are washed removing unbound Goat IgG, unbound HRP-Conjugated Goat IgG Antibody or Goat IgG /HRP-Conjugated Goat IgG Antibody complex. HRP-Conjugated Goat IgG Antibody bound to Goat IgG immobilized in the well remains. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of HRP-Conjugated Goat IgG Antibody captured in the well and inversely proportional to the amount of Goat IgG which was contained in the sample or standard.
ELISA Kit Range0.15 ug/mL - 37.5 ug/mL
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020202020
Mean (ug/mL)2.3772.3502.4422.3412.4052.288
SD0.0210.0260.0270.0360.0410.044
CV%3.3284.1014.3275.6696.5296.864
ELISA Kit Component
ComponentAmount
Goat IgG Microplate96 Well (12 x 8 Well Strips)
Goat IgG Standard6 x 500 uL
100X Anti-Goat IgG Antibody HRP Conjugate1 x 6 mL
Sample Diluent2 x 20 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma
Sensitivity< 0.15 ug/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of goat IgG. No significant cross-reactivity or interference between goat IgG and analogues
was observed.
Assay InfoAssay Methodology: Quantitative Competitive Immunoassay
Gene SymbolGoat IgG