Angiotensin ELISA Kit (Goat)
Katalog-Nummer OKEH04795
Size : 96Wells
Marke : Aviva Systems Biology
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Telefonnummer : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Goat | ||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Angiotensin ELISA Kit (Goat) (OKEH04795) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-goat Angiotensin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Angiotensin and incubated. The Angiotensin found in the sample or standards competes with the biotinylated Angiotensin for limited binding sites on the immobilized anti-goat Angiotensin antibody. Excess unbound biotinylated Angiotensin and sample or standard Angiotensin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Angiotensin captured in the well and inversely proportional to the amount of Angiotensin which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 31.2 - 2,000 pg/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-Assay CV: < 10% Mean Inter-Assay CV: < 12% | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids. | ||||||||||||||||||||||
| Specificity | This assay recognizes recombinant and natural Goat IL-18. No significant cross-reactivity or interference was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive Inhibition Immunoassay |
| Gene Symbol | ANGIOTENSIN |
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| Protein Name | Interleukin-18 |
| Molecular Weight | kDa |