SAA1 ELISA Kit (Horse)
Katalog-Nummer OKIA00045
Size : 1x96wellplate
Marke : Aviva Systems Biology
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Horse | ||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||
ELISA Kit Duration | 110 Minutes | ||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology SAA1 ELISA Kit (Horse) (OKIA00045) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for SAA1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-SAA1 - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SAA1 captured in the well. | ||||||||||||||||
ELISA Kit Range | 2.25 ng/ml - 72 ng/ml | ||||||||||||||||
ELISA Kit Component |
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Additional Information | Intended Use: The SAA test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SAA in Horse biological samples. If the ELISA is to be used outside the intended use, the user may need to optimize for said use. | ||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
Sensitivity | 0.962 ng/ml | ||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Alias Symbols | Serum amyloid A-1 protein, SAA |
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Protein Name | SAA1 |
Uniprot ID | P19857 |