INS ELISA Kit (Horse)

Katalog-Nummer OKCA00593

Size : 96Wells

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHorse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
1:1,0009996-102
1:2,0008784-91
1:4,0009590-99
1:8,0009894-102
ELISA Kit PrincipleAviva Systems Biology INS ELISA Kit (Horse) (OKCA00593) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for INS has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for INS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample INS captured in the well.
ELISA Kit Range1.56 nIU/mL-100 nIU/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)8884-92
EDTA Plasma (n=4)104100-108
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level INS were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level INS were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
INS Microplate96 Wells (12 x 8 Well strips)
INS Lyophilized Standard2
100X Biotinylated INS Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.39 nIU/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
  • Reconstitution & Storage Instructions
  • Western Blotting/Immunoblotting (WB/IB) Protocol
  • Immunohistochemistry (IHC) Protocol
  • Immunocytochemistry (ICC) Protocol
  • Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
  • Blocking Peptide Competition Protocol (BPCP)
  • Immunoprecipitation (IP) Protocol
  • Antibody Array (AA) Protocol
Gene SymbolINS
Gene Full NameInsulin
Protein NameInsulin
Description of TargetInsulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Uniprot IDP01310