INS ELISA Kit (Horse)
Katalog-Nummer OKCA00593
Size : 96Wells
Marke : Aviva Systems Biology
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Horse | ||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology INS ELISA Kit (Horse) (OKCA00593) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for INS has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for INS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample INS captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 1.56 nIU/mL-100 nIU/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision: 3 samples with known low, middle and high level INS were tested with 20 replicates across one plate. Inter-assay Precision: 3 samples with known low, middle and high level INS were tested on three different plates with 8 replicates, respectively. Intra-Assay CV: <8% Inter-Assay CV: <10% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
Sensitivity | 0.39 nIU/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | INS |
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Gene Full Name | Insulin |
Protein Name | Insulin |
Description of Target | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. |
Uniprot ID | P01310 |