IL4 ELISA Kit (Dog)

Katalog-Nummer OKEH00131

Size : 96T

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityCanis lupus, Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL4 ELISA Kit (Dog) (OKEH00131) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL4 captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 8.1% (n = 20)
Mean Inter-assay CV%: < 10.3% (n = 20)
ELISA Kit Component
ComponentAmount
IL4 Microplate96 Wells (12 x 8 Well strips)
IL4 Lyophilized Standard2
100X Biotinylated IL4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity11.2 pg/mL
SpecificityNatural and recombinant Dog Interleukin-4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL4
Gene Full Nameinterleukin 4
Alias SymbolsB-cell stimulatory factor 1;BSF-1;IL-4;interleukin-4;lymphocyte stimulatory factor 1.
NCBI Gene Id403785
Protein NameO77762
Description of TargetInterleukin-4
Uniprot IDhttps://www.uniprot.org/uniprot/O77762
Protein Accession #NP_001003159
Nucleotide Accession #NM_001003159
Protein Size (# AA)132
Molecular Weight15 kDa