GH1 ELISA Kit (Dog)
Katalog-Nummer OKEH00002
Size : 96Wells
Marke : Aviva Systems Biology
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Canis lupus, Dog | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology GH1 ELISA Kit (Dog) (OKEH00002) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-dog GH1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GH1 and incubated. The GH1 found in the sample or standards competes with the biotinylated GH1 for limited binding sites on the immobilized anti-dog GH1 antibody. Excess unbound biotinylated GH1 and sample or standard GH1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GH1 captured in the well and inversely proportional to the amount of GH1 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.625-40ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 5.3% (n = 20) Mean Inter-assay CV%: < 8.5% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.269 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Dog Somatotropin | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | GH1 |
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Gene Full Name | growth hormone |
Alias Symbols | GH;GHB1;Growth hormone;growth hormone 1;growth hormone B1;somatotropin. |
NCBI Gene Id | 403795 |
Protein Name | P33711 |
Description of Target | Somatotropin |
Uniprot ID | https://www.uniprot.org/uniprot/P33711 |
Protein Accession # | NP_001561 |
Nucleotide Accession # | NM_001570 |
Protein Size (# AA) | 625 |
Molecular Weight | 69 kDa |