EPO ELISA Kit (Dog)
Katalog-Nummer OKEH00043
Size : 96T
Marke : Aviva Systems Biology
Contact local distributor :
Telefonnummer : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Canis lupus, Dog | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology EPO ELISA Kit (Dog) (OKEH00043) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EPO has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EPO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EPO captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 3.5% (n = 20) Mean Inter-assay CV%: < 7.6% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.051 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Dog Erythropoietin | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | EPO |
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| Gene Full Name | erythropoietin |
| Alias Symbols | erythropoietin;LOW QUALITY PROTEIN: erythropoietin. |
| NCBI Gene Id | 404002 |
| Protein Name | P33707 |
| Description of Target | Erythropoietin |
| Uniprot ID | https://www.uniprot.org/uniprot/P33707 |
| Protein Accession # | NP_001006647 |
| Nucleotide Accession # | NM_001006646 |
| Protein Size (# AA) | 206 |
| Molecular Weight | 23 kDa |