Ovotransferrin ELISA Kit (Chicken)

Katalog-Nummer OKIA00019

Size : 1kit

Marke : Aviva Systems Biology

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Telefonnummer : +1 850 650 7790
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKIA00019
Product Info
Predicted Species ReactivityChicken
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration60 Minutes
ELISA Kit PrincipleAviva Systems Biology Ovotransferrin ELISA Kit (Chicken) (OKIA00019) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Ovotransferrin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Ovotransferrin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ovotransferrin captured in the well.
ELISA Kit Range6.25 ng/ml - 400 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Chicken Ovotransferrin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Chicken Ovotransferrin Calibrator1
Additional InformationIntended Use: The total Chicken Ovotransferrin (TX) test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Ovotransferrin in biological samples of Chickens.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Chicken Ovotransferrin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-CHICKEN OVOTRANSFERRIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Chicken Ovotransferrin.
7. CHICKEN OVOTRANSFERRIN CALIBRATOR
One vial containing Chicken Ovotransferrin calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity1.473 ng/ml
Predicted Homology Based on Immunogen SequenceChicken
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTF
Gene Full Nametransferrin (ovotransferrin)
Alias SymbolsLTF, TFEW, conalbumin
NCBI Gene Id396241
Protein NameOvotransferrin
Description of TargetTransferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. Responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. There are two forms of hen transferrin, ovotransferrin, found in the ovoducts and, serum transferrin, secreted by the liver. Serum transferrin may also have a role in stimulating cell proliferation and is regulated by iron levels. Ovotransferrin has a bacteriostatic function and, is not controlled by iron levels.
Uniprot IDP02789