Application
- For evaluation and high-throughput screen (HTS) of nitric oxide synthase (NOS) modulators.
Key Features
- High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system.
- Rapid and reliable. Can be completed in less than 3 hours if the assay is performed at 37°C.
Method
- OD540nm
Samples
- Nitric Oxide Synthase
Species
- All
Procedure
- 2-3 hours
Size
- 100 tests
Shelf Life
- 6 months
More Details
Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO is the oxidation product of arginine by nitric oxide synthase (NOS) and is involved in host defense development, activation of regulatory proteins, and direct covalent interaction with functional biomolecules. Inhibition of NOS has the potential to produce diverse biological effects, particularly in the cardiovascular system. Simple, direct, and non-radioactive procedures for measuring NOS are becoming popular in research and drug discovery. BioAssay Systems EnzyChrom™ Nitric Oxide Synthase Inhibitor Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following the reduction of nitrate to nitrite using an improved Griess method.No frequently asked questions for this new product. Please check back later.
For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.
No citations for this new product. Please check back later. You may click here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost