Cellular Glutathione Peroxidase Assay Kit

Katalog-Nummer FR17

Size : Kit

Marke : Oxford Biomedical

Contact local distributor :


Telefonnummer : +1 850 650 7790

Product Overview

Background

Cellular glutathione peroxidase (c-GPx) is a member of a family of GPx enzymes whose function is to detoxify peroxides in the cell, thus playing a critical role in protecting the cell from free radical damage, particularly lipid peroxidation. This assay provides an indirect measure of the activity of c-GPx. Oxidized glutathione (GSSG), produced upon reduction of an organic peroxide by c-GPx, is recycled to its reduced state by the enzyme glutathione reductase (GPx). The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm, thus providing a spectrophotometric means for monitoring GPx enzymes activity. The rate decrease in the A340 is directly proportional to the GPx activity in the sample.

Recommended References

  1. F. Ursini, et. al. (1995)  Meth. in Enzymol. 252:38-53.
  2. F.F. Chu (1994) Cytogenet. Cell Genet. 66:96-98.
  3. G.C. Mills (1957) J. Biol. Chem. 229:89-197.
  4. F.F. Chu, et. al. (1993)  J. Biol. Chem. 268:2571-2576.
  5. K.T. Takahashi, et. al. (1987) Arch. Biochm. Biophys. 256:677-686.
  6. F. Ursini, et. al. (1982) Biochm. Biophys. Acta. 710:97-211.
  7. D.E. Paglia, and W.N. Valentine (1967) J. Lab. Clin. Med. 70:158-169.
  8. M.K. Cha, and I.H. Kim (1996) Biochem. Biophys. Res. Commun. 222:619-625.

 

MSDS
Spec Sheet