Phenylethanolamine A ELISA BioAssay™ Kit

Katalog-Nummer 376909-96T

Size : 96Tests

Marke : US Biological

Contact local distributor :


Telefonnummer : +1 850 650 7790


Applications
E

Shipping Temp
Blue Ice

Storage Temp
4°C/-20°C

Phenylethanolamine A, named as Phenethylamine, can accelerate animal growth, decrease fat deposition, increase the amount of lean meat as well as Ractopamine. Phenylethanolamine A is used as a new type of Clenbuterol and widely used on animal production. The general symptoms are nausea, dizziness, asthenia and fibrillation after eating high-Phenylethanolamine A-food, even chromosome aberration and cancer. Thus Phenylethanolamine A is listed as “Prohibit material within animal and water” on No.1519 from Ministry of Agriculture of the People's Republic of China. This BioAssay™ kit is a detection product developed based on ELISA technology, with operation time as short as 50 minutes.

Sample Type:
Urine

Intended Use:
This BioAssay™ kit allows for the in vitro quantitative determination of Phenylethanolamine A concentrations in urine.

Sensitivity:
0.125ppb

Range:
0.125-2ppb

Precision:
Intra-assay: CV%<10%
Inter-assay: CV%<10%

Test Principle:
This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay™ kit has been pre-coated with Phenylethanola mine A antigen. Standards or samples are added to the appropriate microtiter plate wells with Phenylethanolamine A specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Phenylethanolamine A and Phenylethanolamine A in standards or samples with the Phenylethanolamine A special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Phenylethanolamine A in the standards or samples. The color development is stopped and the intensity of the color is measured.

Kit Components:
Assay plate, 96T/48T
Standard (96T), 6x1ml
Standard (48T), 6x0.5ml
HRP-conjugate (96T), 1x7ml
HRP-conjugate (48T), 1x3.5ml
Antibody (96T), 1x7ml
Antibody (48T), 1x1x3.5ml
TMB Substrate (96T), 1x12ml
TMB Substrate (48T), 1x6ml
Stop Solution (96T), 1x10ml
Stop Solution, (48T), 1x5ml
Wash Buffer (10X) (96T), 1x50ml
Wash Buffer (10X) (48T), 1x25ml
Adhesive Strip (96T/48T), 4x strips
Instruction Manual (96T/48T), 1x manual

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C.
Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.