Glutathione Peroxidase Assay Kit, BioAssay™

Katalog-Nummer G8127-03-96T

Size : 96Tests

Marke : US Biological

Contact local distributor :


Telefonnummer : +1 850 650 7790


Shipping Temp
Dry Ice

Storage Temp
-20°C

Glutathione Peroxidase (GPX, EC 1.11.1.9) represents an enzyme family with peroxidase activity whose main biological role is to protect the organism from oxidative damage. It helps prevent lipid peroxidation of cellular membranes by removing free peroxide in the cell. GPX catalyzes the following reaction with glutathione reductase (GR):

GPX GR
2GSH + ROOH -----> GSSG + ROH + H2O, GSSG + NADPH ---->
2GSH + NADP+

Simple, direct and high-throughput assays for GPX activity find wide Applications. The improved assay directly measures NADPH consumption in the enzyme coupled reactions. The measured decrease in optical density at 340nm is directly proportional to the enzyme activity in the sample.

Key Features:
Sensitive and accurate: Use 10ul sample
Linear detection range: 40-800U/L GPX activity

Applications:
Direct Assays: GPX activity in biological samples
Drug Discovery/Pharmacology: Effects of drugs on GPX activity

Kit Components: (100 Tests In 96-Well Plates):
G8127-03A: Assay Buffer, 1x25ml
G8127-03B: GR Enzyme, 1x250ul
*G8127-03C: Glutathione, 1x1vial
*G8127-03D: NADPH, 1x1vial
G8127-03E: H2O2 Solution, 1x50ul
*G8127-03F: Calibrator, 1x1vial

Storage and Stability:
Store all components at -20°C. After reconstitution **G8127-03C, *G8127-03D and *G8127-03F are stable for 3 weeks. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.