Hif1a Mouse Gene Knockout Kit (CRISPR)
CAT#: KN307708LP
Hif1a - mouse gene knockout kit via CRISPR, HDR mediated
Functional Cassette: GFP-puro RFP-BSD mBFP-Neo
HDR-mediated knockout kit validation
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Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
| Donor DNA | Luciferase-Puro |
| Symbol | Hif1a |
| Locus ID | 15251 |
| Components | KN307708G1, Hif1a gRNA vector 1 in pCas-Guide CRISPR vector KN307708G2, Hif1a gRNA vector 2 in pCas-Guide CRISPR vector KN307708LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette. GE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_010431 |
| UniProt ID | Q61221 |
| Synonyms | AA959795; bHLHe78; HIF-1-alpha; HIF1-alpha; HIF1alpha; MOP1 |
| Summary | This gene encodes the alpha subunit which, along with the beta subunit, forms a heterodimeric transcription factor that regulates the cellular and developmental response to reduced oxygen tension. The transcription factor has been shown to regulate genes involved in several biological processes, including erythropoiesis and angiogenesis which aid in increased delivery of oxygen to hypoxic regions. The transcription factor also plays a role in the induction of genes involved in cell proliferation and survival, energy metabolism, apoptosis, and glucose and iron metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] |
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Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN307708 | Hif1a - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN307708BN | Hif1a - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN307708RB | Hif1a - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN507708 | Hif1a - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA201899 | Hif1a CRISPRa kit - CRISPR gene activation of mouse hypoxia inducible factor 1, alpha subunit | 1 657,00 EUR |