Folr1 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN306057RB

Folr1 - mouse gene knockout kit via CRISPR, HDR mediated



HDR-mediated knockout kit validation

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Specifications

Product Data
Format 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA RFP-BSD
Symbol Folr1
Locus ID 14275
Components

KN306057G1, Folr1 gRNA vector 1 in pCas-Guide CRISPR vector

KN306057G2, Folr1 gRNA vector 2 in pCas-Guide CRISPR vector

KN306057RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001252552, NM_001252553, NM_001252554, NM_008034
UniProt ID P35846
Synonyms FBP1; Folbp-1; Folbp1
Summary Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. Required for normal embryonic development and normal cell proliferation. Required for renal folate reabsorption.[UniProtKB/Swiss-Prot Function]

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