full length recombinant ycf3 protein of Chlamydomonas reinhardtii UniProt: O20031, as described in Boudreau et al. 1997
Host:
Rabbit
Clonality:
Polyclonal
Purity:
Serum
Format:
Lyophilized
Quantity:
200 µl
Reconstitution:
For reconstitution add 200 µl of sterile water
Storage:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:
Western blot (WB)
Recommended dilution:
1 : 1000 (WB)
Expected | apparent MW:
19 kDa
Reactivity
Confirmed reactivity:
Chlamydomonas reinhardtii, cyanobacteria
Predicted reactivity:
Algae, Arabidopsis thaliana, Avena sativa, Chlorella vulgaris, Marchantia polymorpha, Phaseolus vulgaris, Physcomitrium patens, Chlorokybus atmophyticus, Ostreococcus tauri Species of your interest not listed? Contact us
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Additional Information
Additional information (application):
Western blot detection image can be found in Boudreau et al. 1997.
Background
Background:
Ycf3 together with Ycf4 have been found as extrinistic thylakoid membrane proteins which are required for the accumulation of PSI complex in Chlamydomonas reinhardii.
Product Citations
Selected references:
Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113. Epub 2016 Feb 22. Naver et al. (2001). Functional studies of Ycf3. The Plant Cell 13:2731- 2746. Boudreau et al. (1997) The chloroplast ycf3 and ycf4 open reading frames of Chlamydomonas reinhardtii are required for the accumulation of the photosystem I complex. The EMBO J.16:6095-6104.
Protocols
Agrisera Western Blot protocol and video tutorials
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