Y-27632 (dihydrochloride) [129830-38-2]
Katalog-Nummer HY-10583-50mg
Size : 50mg
Marke : MedChemExpress
Dichlorhydrate Y-27632 est un inhibiteur de ROCK-I et de ROCK-II qui est actif par voie orale et ATP-compétitif, avec Kis de 220 et 300 nM, respectivement. Dichlorhydrate Y-27632 induit l'apoptose. Dichlorhydrate Y-27632 amorce les cellules souches pluripotentes induites par l'homme (hIPSC) pour se différencier sélectivement vers la lignée mésendodermique via une modulation de type transition épithéliale-mésenchymateuse.
Y-27632 Dihydrochlorid ist ein oral wirksamer, ATP-kompetitiver Inhibitor von ROCK-I und ROCK-II mit einem Kis-Wert von 220 bzw. 300 nM. Y-27632 Dihydrochlorid induziert apoptosis. Y-27632 Dihydrochlorid veranlasst humane induzierte pluripotente Stammzellen (hIPSCs), sich selektiv über epithelial-mesenchymale Übergangs-ähnliche Modulation in Richtung mesendodermale Abstammung zu differenzieren.
Y-27632 dihydrochloride is an orally active and ATP-competitive ROCK (Rho-kinase) inhibitor (ROCK-I Ki=220 nM; ROCK-II Ki=300 nM). Y-27632 dihydrochloride shows antiepileptic effects.
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Y-27632 dihydrochloride Chemische Struktur
CAS. Nr. : 129830-38-2
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- Immunofluorescence imaging of LNCaP cells treated with the Cytoskeleton B (CB), Nocodazole (NO), PF-573288 (PF), Y-27632 (Y), and (-)-Blebbistatin ((-) Bl). After the cells are cultured on different substrates for 3 days, inhibitors are added and incubated for 24 h.
- Expression of CTC cluster-related (plakoglobin and CD44) and EMT-related (vimentin and E-cadherin, 4 days) proteins in LNCaP cells grown on different substrates after treatment with different inhibitors (Cytoskeleton B (CB), Nocodazole (NO), PF-573288 (PF), Y-27632 (Y), and (-)-Blebbistatin ((-) Bl)).
- MHCC-97H and Huh-7 cells co-transfected with control or supervillin-specific siRNA and a RhoA(WT), RhoA(V14), or RhoA(N19) plasmids for 48 h are treated with PBS or Y27632 2HCl (10 μM) for 16 h during hypoxia, and then assayed for phosphorylated p38 and ERK by immunoblotting. β-actin is used as the loading control
- SKOV3 mock-transfected control (Ctrl) and shRNA-Hic-5 knockdown (KD) (sh-S1 and sh-S2) cells were either untreated (-) or treated (+) with 10mM Y27632 over a period of 48 hr. SKOV3 mock-transfected control (Ctrl) and shRNA-Hic-5 knockdown (KD) (sh-S1 and sh-S2) clones were either untreated (-) or treated (+) with 10 mM Y27632 followed by stimulation without (-) or with (+) 5 ng/mL of TGFβ1 for 48 hr.
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Y-27632 dihydrochloride is an orally active and ATP-competitive ROCK (Rho-kinase) inhibitor (ROCK-I Ki=220 nM; ROCK-II Ki=300 nM). Y-27632 dihydrochloride shows antiepileptic effects. | |||||||||||||||||
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In Vitro | Y-27632 (1-5 μM; 0-60 min) promotes neuronal differentiation of adipose tissue-derived stem cells (ADSCs) . MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot Analysis
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In Vivo | Y-27632 (oral gavage; 30 mg/kg; once daily; 4 w) prevents dimethylnitrosamine-induced hepatic fibrosis in rats. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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320.26 | |||||||||||||||||
C14H23Cl2N3O | |||||||||||||||||
129830-38-2 | |||||||||||||||||
Solid | |||||||||||||||||
White to off-white | |||||||||||||||||
O=C([C@@H]1CC[C@]([C@H](N)C)(16)CC1)NC2=CC=NC=C2.16Cl.16Cl | |||||||||||||||||
Room temperature in continental US; may vary elsewhere. | |||||||||||||||||
4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) | |||||||||||||||||
In Vitro: H2O : 100 mg/mL (312.25 mM; Need ultrasonic) DMSO : 33.33 mg/mL (104.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing Stock Solutions
View the Complete Stock Solution Preparation Table * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo: Select the appropriate dissolution method based on your experimental animal and administration route. For the following dissolution methods, please ensure to first prepare a clear stock solution using an and then sequentially add co-solvents:
For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator Please enter the basic information of animal experiments: Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments. Calculation results: Working solution concentration: mg/mL This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details. The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE). | |||||||||||||||||
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[1]. Tada S, et al. A selective ROCK inhibitor, Y27632, prevents dimethylnitrosamine-induced hepatic fibrosis in rats. J Hepatol. 2001 Apr;34(4):529-36.
[2]. Inan S, et al. Antiepileptic effects of two Rho-kinase inhibitors, Y-27632 and fasudil, in mice. Br J Pharmacol. 2008 Sep;155(1):44-51.
[3]. Xue ZW, et al. Rho-associated coiled kinase inhibitor Y-27632 promotes neuronal-like differentiation of adult human adipose tissue-derived stem cells. Chin Med J (Engl). 2012 Sep;125(18):3332-5.
[4]. Ishizaki T, et al. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol. 2000 May;57(5):976-83.
* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO / H2O | 1 mM | 3.1225 mL | 15.6123 mL | 31.2246 mL | 78.0616 mL |
5 mM | 0.6245 mL | 3.1225 mL | 6.2449 mL | 15.6123 mL | |
10 mM | 0.3122 mL | 1.5612 mL | 3.1225 mL | 7.8062 mL | |
15 mM | 0.2082 mL | 1.0408 mL | 2.0816 mL | 5.2041 mL | |
20 mM | 0.1561 mL | 0.7806 mL | 1.5612 mL | 3.9031 mL | |
25 mM | 0.1249 mL | 0.6245 mL | 1.2490 mL | 3.1225 mL | |
30 mM | 0.1041 mL | 0.5204 mL | 1.0408 mL | 2.6021 mL | |
40 mM | 0.0781 mL | 0.3903 mL | 0.7806 mL | 1.9515 mL | |
50 mM | 0.0624 mL | 0.3122 mL | 0.6245 mL | 1.5612 mL | |
60 mM | 0.0520 mL | 0.2602 mL | 0.5204 mL | 1.3010 mL | |
80 mM | 0.0390 mL | 0.1952 mL | 0.3903 mL | 0.9758 mL | |
100 mM | 0.0312 mL | 0.1561 mL | 0.3122 mL | 0.7806 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
- Cell Cycle/DNA Damage TGF-beta/Smad Stem Cell/Wnt Cytoskeleton
- Organoid ROCK
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
Y-27632129830-38-2Y27632Y 27632OrganoidROCKRho-associated protein kinaseRho-associated kinaseRho-kinaseROKantiepileptic effectadipose tissue-derived stem cellsADSCsInhibitorinhibitorinhibit
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- Produktname:
- Y-27632 dihydrochloride
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