General Info
| Applications: | ELISA |
| Reactivity: | All |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.38pg/mL |
| Detection Limit: | 15.63~1000pg/mL |
| Short Description: | This VK1 Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in all cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 2.5h |
| Detection: | Colormetric |
Information
| Specificity: | This kit recognizes VK1 in samples. No significant cross-reactivity or interference between VK1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Background | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VK1. During the reaction, VK1 in the sample or standard competes with a fixed amount of VK1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VK1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VK1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance