Top1 (NM_009408) Mouse Tagged ORF Clone
CAT#: MR218547
- TrueORF®
Top1 (Myc-DDK-tagged) - Mouse topoisomerase (DNA) I (Top1). Note: ORF is codon optimized
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
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Product Images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Top1 |
| Synonyms | AI467334; D130064I21Rik; Top-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | >MR218547 representing NM_009408, codon optimized. Due to the complexity of NM_009408, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGGTGACCATCTGCATAATGATTCCCAGATCGAAGCTGATTTTAGACTGAATGACAGTCATAAAC ACAAAGACAAGCATAAGGATAGAGAACATCGGCACAAGGAGCACAAGAAAGATAAGGATAAAGACAGAGA AAAATCCAAGCACTCCAATTCCGAGCATAAGGATAGTGAGAAGAAACACAAGGAGAAAGAAAAAACAAAA CATAAAGATGGTAGCAGTGAGAAACACAAGGACAAGCACAAGGATCGGGATAAAGAGCGCAGAAAAGAGG AAAAGATTAGGGCGGCCGGTGACGCGAAGATTAAGAAAGAGAAAGAGAATGGCTTTTCTTCTCCACCACG CATCAAGGACGAGCCCGAAGATGATGGGTACTTTGCTCCGCCCAAGGAGGATATTAAGCCTCTGAAACGG CTCAGGGACGAGGATGACGCCGACTATAAACCAAAAAAAATAAAGACCGAAGACATAAAGAAAGAGAAGA AGAGAAAAAGTGAGGAAGAAGAGGATGGAAAGCTCAAAAAGCCAAAAAACAAGGATAAGGATAAAAAGGT AGCAGAGCCTGATAACAAGAAGAAGAAGCCAAAAAAAGAAGAGGAACAAAAGTGGAAATGGTGGGAAGAG GAACGCTATCCCGAAGGCATAAAGTGGAAGTTTCTGGAACATAAGGGTCCTGTGTTCGCTCCTCCTTACG AACCACTGCCTGAAAGCGTCAAATTTTACTACGACGGGAAGGTCATGAAACTCTCCCCTAAAGCGGAGGA GGTAGCTACTTTTTTCGCAAAGATGCTCGACCATGAGTACACAACTAAGGAGATCTTCCGGAAAAACTTT TTCAAAGATTGGAGAAAGGAGATGACTAATGACGAGAAGAATACCATCACAAATCTGTCTAAGTGCGACT TTACCCAAATGTCTCAGTATTTTAAGGCACAGTCTGAGGCTAGGAAGCAGATGAGTAAGGAGGAAAAGCT CAAGATCAAGGAGGAAAATGAGAAGCTGCTGAAGGAGTATGGATTTTGTGTCATGGACAATCACCGGGAA CGCATCGCCAACTTCAAGATCGAACCTCCTGGACTGTTTCGGGGGCGCGGGAATCATCCAAAAATGGGTA TGCTGAAGCGCCGAATCATGCCAGAGGATATCATCATCAACTGCAGTAAAGATGCCAAGGTTCCCAGCCC TCCGCCCGGGCACAAATGGAAGGAAGTGCGGCACGATAACAAGGTGACGTGGCTCGTCAGCTGGACAGAA AACATCCAGGGCTCAATCAAATATATTATGCTGAATCCTTCCTCCCGAATCAAAGGAGAGAAGGACTGGC AGAAGTACGAAACCGCACGCCGCCTTAAAAAGTGTGTCGACAAGATTCGCAATCAGTATAGAGAAGATTG GAAATCCAAAGAAATGAAGGTCCGCCAAAGGGCTGTGGCTTTGTACTTCATAGACAAGCTGGCTTTGCGA GCCGGGAACGAGAAGGAAGAAGGCGAGACCGCAGACACTGTGGGGTGCTGCTCTCTGCGGGTTGAGCATA TCAATCTGCATCCAGAGCTCGATGGGCAAGAGTACGTGGTCGAGTTTGATTTCCCCGGGAAGGATAGCAT TCGATACTACAACAAGGTGCCTGTGGAAAAACGGGTGTTCAAGAATCTTCAGCTGTTCATGGAGAATAAA CAGCCAGAGGATGATCTCTTTGACAGACTGAACACTGGGATCCTGAACAAGCACCTGCAGGACTTGATGG AAGGGCTCACAGCTAAGGTCTTTCGGACTTACAACGCCAGTATTACCCTGCAGCAGCAGCTTAAGGAGCT GACAGCCCCAGATGAAAATGTCCCAGCAAAGATCCTTTCATACAACCGCGCAAACAGGGCAGTAGCCATC CTGTGTAATCATCAACGGGCCCCACCCAAAACGTTTGAAAAGAGCATGATGAATTTGCAGTCTAAAATAG ATGCCAAGAAGGACCAGCTGGCAGACGCCAGGCGAGATCTTAAGAGCGCTAAGGCTGACGCCAAAGTGAT GAAAGACGCTAAGACCAAAAAGGTGGTGGAATCCAAGAAGAAGGCAGTACAGAGGCTGGAAGAACAGCTG ATGAAGCTCGAGGTGCAAGCCACCGATCGCGAGGAGAACAAGCAGATAGCCCTGGGGACTAGTAAGCTGA ATTACCTTGACCCAAGGATCACCGTGGCTTGGTGCAAAAAATGGGGCGTGCCCATCGAGAAAATCTATAA CAAAACTCAGCGGGAGAAATTCGCATGGGCTATCGACATGACCGATGAAGACTACGAATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218547 representing NM_009408 Red=Cloning site Green=Tags(s) MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKDKDKDREKSKHSNSEHKDSEKKHKEKEKTK HKDGSSEKHKDKHKDRDKERRKEEKIRAAGDAKIKKEKENGFSSPPRIKDEPEDDGYFAPPKEDIKPLKR LRDEDDADYKPKKIKTEDIKKEKKRKSEEEEDGKLKKPKNKDKDKKVAEPDNKKKKPKKEEEQKWKWWEE ERYPEGIKWKFLEHKGPVFAPPYEPLPESVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNF FKDWRKEMTNDEKNTITNLSKCDFTQMSQYFKAQSEARKQMSKEEKLKIKEENEKLLKEYGFCVMDNHRE RIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTE NIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALR AGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENK QPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENVPAKILSYNRANRAVAI LCNHQRAPPKTFEKSMMNLQSKIDAKKDQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQL MKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMTDEDYEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_009408 |
| ORF Size | 2301 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@biotrend.com The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009408.2, NP_033434.2 |
| RefSeq Size | 3859 bp |
| RefSeq ORF | 2304 bp |
| Locus ID | 21969 |
| UniProt ID | Q04750 |
| Cytogenetics | 2 80.96 cM |
| MW | 90.9 kDa |
| Gene Summary | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| MC221438 | Top1 (untagged) - Mouse topoisomerase (DNA) I (Top1), (10ug) | 773,00 EUR | |
| MG218547 | Top1 (tGFP-tagged) - Mouse topoisomerase (DNA) I (Top1), (10ug). | 1 253,00 EUR | |
| MR218547L3 | Lenti ORF clone of Top1 (Myc-DDK-tagged) - Mouse topoisomerase (DNA) I (Top1). Note: ORF is codon optimized | 1 353,00 EUR | |
| MR218547L4 | Lenti ORF clone of Top1 (mGFP-tagged) - Mouse topoisomerase (DNA) I (Top1). Note: ORF is codon optimized | 1 353,00 EUR |