TNF-alpha, Recombinant, Mouse, Animal Free

NF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms; a type II transmembrane protein, and a mature soluble protein.

The TNF-α transmembrane protein is proteolitically cleaved to yield a soluble, biologically active, 17kD TNF-α, which forms a non-covalently linked homotrimer in solution.

Recombinant protein corresponding to mouse TNF-alpha, a soluble 157 amino acid protein (17.3kD) which corresponds to C-terminal extracellular domain of the full length transmembrane protein, expressed in E. coli.

Determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D. The expected ED50 is <0.1ng/ml, corresponding to a specific activity of >1x10e7units/mg.

MLRSSSQNS SDKPVAHVV ANHQVEEQL EWLSQRANA LLANGMDLK DNQLVVPAD GLYLVYSQV LFKGQGCPD YVLLTHTVS RFAISYQEK VNLLSAVKS PCPKDTPEG AELKPWYEP IYLGGVFQL EKGDQLSAE VNLPKYLDF AESGQVYFG VIAL

Verified by UV Spectroscopy and/or SDS-PAGE gel.

Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant, E. coli

≥98% (SDS-PAGE gel, HPLC). Endotoxin

~0.1mg/ml (after reconstitution)

Supplied as a lyophilized powder from 10mM sodium phosphate pH 7.5 Reconstitute with sterile dH2O PBS to 0.1-1mg/ml. Do not vortex.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.