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Sandwich ELISA (Recombinant protein)
Application

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged TGIF is approximately 0.1ng/ml as a capture antibody.

Protocol Download
  • Specification

    Product Description

    Mouse monoclonal antibody raised against a partial recombinant TGIF.

    Immunogen

    TGIF (NP_003235, 163 a.a. ~ 272 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

    Sequence

    PGSVLARPSVICHTTVTALKDVPFSLCQSVGVGQNTDIQQIAAKNFTDTSLMYPEDTCKSGPSTNTQSGLFNTPPPTPPDLNQDFSGFQLLVDVALKRAAEMELQAKLTA

    Host

    Mouse

    Reactivity

    Human

    Interspecies Antigen Sequence

    Mouse (89); Rat (85)

    Isotype

    IgG1 Kappa

    Quality Control Testing

    Antibody Reactive Against Recombinant Protein.

    Storage Buffer

    In 1x PBS, pH 7.4

    Storage Instruction

    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Sandwich ELISA (Recombinant protein)

    Detection limit for recombinant GST tagged TGIF is approximately 0.1ng/ml as a capture antibody.
    Protocol Download

    ELISA

  • Gene Info — TGIF1

    Entrez GeneID

    7050

    GeneBank Accession#

    NM_003244

    Protein Accession#

    NP_003235

    Gene Name

    TGIF1

    Gene Alias

    HPE4, MGC39747, MGC5066, TGIF

    Gene Description

    TGFB-induced factor homeobox 1

    Omim ID

    142946 602630 605389

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and eight variants, encoding four distinct isoforms, are described. [provided by RefSeq

    Other Designations

    5'-TG-3' interacting factor|TALE homeobox TG-interacting factor|TG-interacting factor|homeobox protein TGIF|transforming growth factor-beta-induced factor

  • Interactome
  • Disease