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Sandwich ELISA (Recombinant protein)
Application

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged TFF3 is approximately 0.1ng/ml as a capture antibody.

Protocol Download
QC Test

Western Blot detection against Immunogen (32.23 KDa) .

  • Specification

    Product Description

    Mouse monoclonal antibody raised against a full length recombinant TFF3.

    Immunogen

    TFF3 (AAH17859.1, 15 a.a. ~ 73 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

    Sequence

    EEYVGLSANQCAVPAKDRVDCGYPHVTPKECNNRGCCFDSRIPGVPWCFKPLQEAECTF

    Host

    Mouse

    Reactivity

    Human

    Interspecies Antigen Sequence

    Mouse (78); Rat (76)

    Isotype

    IgG1 Kappa

    Quality Control Testing

    Antibody Reactive Against Recombinant Protein.

    Western Blot detection against Immunogen (32.23 KDa) .

    Storage Buffer

    In 1x PBS, pH 7.4

    Storage Instruction

    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot (Recombinant protein)

    Protocol Download

    Sandwich ELISA (Recombinant protein)

    Detection limit for recombinant GST tagged TFF3 is approximately 0.1ng/ml as a capture antibody.
    Protocol Download

    ELISA

  • Gene Info — TFF3

    Entrez GeneID

    7033

    GeneBank Accession#

    BC017859

    Protein Accession#

    AAH17859.1

    Gene Name

    TFF3

    Gene Alias

    HITF, ITF, TFI, hP1.B

    Gene Description

    trefoil factor 3 (intestinal)

    Omim ID

    600633

    Gene Ontology

    Hyperlink

    Gene Summary

    Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq

    Other Designations

    intestinal trefoil factor|secretory protein|trefoil factor 3|trefoil factor 3, HITF, human intestinal trefoil factor

  • Interactome
  • Disease
  • Publication Reference