Tau (Neurofibrillary Tangles Marker, Microtubule-associated Protein, MAP, Paired helical filament-tau, PHF-tau)
Katalog-Nummer T1029-100ug
Size : 100ug
Marke : US Biological
Host
mouseIsotype
IgG1Clone Number
2B2.100Grade
PurifiedApplications
E IC IF IHC IP WBShipping Temp
Blue IceStorage Temp
-20°CTau proteins are important in the assembly of microtubules. In normal brain, Tau is localized within the axons of the neurons, but in some neuropathological lesions it accumulates within the body of the neuron. Tau proteins are thought to be abnormally phosphorylated in certain disease states. Tau has been immunohistochemically localized to neurofibrillary tangles of Alzheimer’s disease (AD) brains. Tau proteins promote the assembly of tubulin monomers into microtubules and stabilize microtubules. Alternate splicing of Tau mRNA, glycosylation, and differential phosphorylation contribute to the heterogeneity of Tau.
Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 1:500
Immunohistochemistry (Paraffin): 1:20-1:200. For formalin-fixed paraffin embedded tissues, staining is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes prior to antibody incubation. Intensely stains the human neurofibrillary tangles, neuropil threads and neuritic plaques associated with Alzheimer’s disease. Observed to stain astrocytes.
Optimal dilutions to be determined by the researcher.
Immunohistochemistry (Paraffin): 1:20-1:200. For formalin-fixed paraffin embedded tissues, staining is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes prior to antibody incubation. Intensely stains the human neurofibrillary tangles, neuropil threads and neuritic plaques associated with Alzheimer’s disease. Observed to stain astrocytes.
Optimal dilutions to be determined by the researcher.
Recommended Positive Control:
Human T98G glioblastoma, SH-SY5Y cells or brain tissue.
Hybridoma:
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Product Type
Mab
Isotype
IgG1
Clone No
2B2.100
Host
mouse
Source
bovine
Concentration
~0.5mg/ml
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified.
Immunogen
Purified bovine microtubule-associated proteins.
Specificity
Recognizes and is highly specific for non-phosphorylated and phosphorylated forms of bovine Tau proteins (45-68kD). Does not crossreact with other microtubule associated proteins (MAPs) or tubulin. The epitope is located in the middle region of Tau.
Species Crossreactivity
human, sheep, mouse and rat.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.