Buffer: PBS containing 10mg/ml BSA Preservative: 0.002% Thimerosal Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Warnings: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Reconstitution and Storage
Short-term (up to 6 months) store at 2 to 8C. Long term, aliquot and store at -20C. Avoid multiple freeze/thaw cycles.
Immunogen
Human RSV isolate, confirmed
Purification
IgG fraction covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed.
Specificity
All RSV viral antigens. Reacts well with bovine isolates. Does not react with Para 1-3, Influenza A & B or Adenovirus by IFA. Negative against HEp-2 cells and WI-38 cells.
Application Info
Suitable for use in ELISA, Immunohistochemistry (paraffin sections) and Neutralizing. Ethanol-fixation is not recommended. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.