Specifications

Product Data
Applications	FC, ICC/IF, Simple Western, WB
Recommended Dilution	Simple Western: 1:25, Immunocytochemistry/ Immunofluorescence: 1:50, Western Blot: 1:2000, Knockout Validated, Flow Cytometry
Reactivities	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal region of the human p62/SQSTM1 protein (within residues 400-450). [Swiss-Prot Q13501]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage	Store at -20°C as received.
Stability	Stable for 12 months from date of receipt.
Predicted Protein Size	47 kDa
Gene Name	sequestosome 1
Database Link	NP_003891 Entrez Gene 18412 MouseEntrez Gene 113894 RatEntrez Gene 8878 Human UniProt ID Q13501
Background	p62/SQSTM1 (sequestosome 1) is ubiquitously-expressed cytoplasmic/adapter protein conserved in vertebrates and it can be induced by proteasomal inhibitor PSI, PGJ2/prostaglandin J2 as well as phorbol 12-myristate 13-acetate (PMA). SQSTM1 has the ability of ubiquitin binding as well as regulating NFkB1 activation by TNF-alpha, NGF (nerve growth factor) and interleukin-1. SQSTM1 acts as an adapter that mediates the interaction between TRAF6 and CYLD, and SQSTM1-TRAF6 interaction leads to K63-linked polyubiquitination of TRAF6 followed by subsequent activation of NFkB pathway. SQSTM1 plays a role in TITIN/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. SQSTM1 is essential both for formation and autophagic degradation of polyubiquitin-containing bodies which are known as aggresome-like induced structures (ALIS) and SQSTM1 connects ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members. Moreover, while autophagy modulates SQSTM1 protein levels, SQSTM1 suppresses autophagy via activation of mTORC1. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress. SQSTM1 functions as a signaling hub for various signal transduction pathways, apoptosis, cell differentiation, apoptosis, immune response, regulation of K+ channels and Nrf2 activation, and its dysregulation is associated with Paget disease of bone and tumorigenesis.
Synonyms	A170; OSIL; p60; p62; p62B; PDB3; ZIP3
Note	This p62/SQSTM1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~47 kDa.
Reference Data
Protein Families	Druggable Genome, Transcription Factors