SNAP-25 Antibody

Katalog-Nummer ASM10122-STR-100

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Marke : Abcepta

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  • IHC - SNAP-25 Antibody ASM10122
    Immunohistochemistry analysis using Mouse Anti-SNAP25 Monoclonal Antibody, Clone SP-12 (ASM10122). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SNAP25 Monoclonal Antibody (ASM10122) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Positive muscle, epidermal and dermal staining.
  • WB - SNAP-25 Antibody ASM10122
    Western Blot analysis of Human Cell lysates showing detection of SNAP25 protein using Mouse Anti-SNAP25 Monoclonal Antibody, Clone SP-12 (ASM10122). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SNAP25 Monoclonal Antibody (ASM10122) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, E, AM
Primary Accession P60880
Other Accession NP_570824.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat, Pig, Cat
Clonality Monoclonal
Description Mouse Anti-Human SNAP-25 Monoclonal IgG1
Target/Specificity Detects ~29kDa.
Other Names Bdr Antibody, RIC4 Antibody, SNAP25 Antibody, SNAP-25B Antibody, SNP25 Antibody, SUP Antibody, super protein Antibody, synaptosomal associated 25kDa protein Antibody
Clone Names SP-12
Immunogen Raised against a crude synaptic preparation from the post mortem human brain
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-180 was sufficient for detection of SNAP-25 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm