Rat IgG1 Antibody : Biotin
Katalog-Nummer OASB00766
Size : 0.5mg
Marke : Aviva Systems Biology
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Telefonnummer : +1 850 650 7790
Rat IgG1 Antibody : Biotin (OASB00766)
| Datasheets/Manuals | Printable datasheet for Rat IgG1 Antibody : Biotin (OASB00766) |
|---|
| Tested Species Reactivity | Rat |
|---|---|
| Predicted Species Reactivity | Rat |
| Clonality | Monoclonal |
| Clone | G11C5 |
| Isotype | IgG2b |
| Host | Mouse |
| Conjugation | Biotin |
| Application | FC, IHC-F, ELISPOT |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Rat IgG1 hybridoma |
| Concentration | 0.5 mg/mL |
| Specificity | IgG1 |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested as a 2nd step reagent by flowcytometry and/or ELISA to conform to characteristics of a standard reference reagent. Representative dataare included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Weareye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled,contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide- containingcompounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: ELISA: Fluorescein conjugate Cy5 conjugate R-phycoerythrin conjugate Biotin conjugate Horseradish Peroxidase conjugate Alkaline Phosphatase conjugate Biotin conjugate <= 1.0 ug/106 cells <= 0.1 ug/106 cells <= 0.1 ug/106 cells <= 1.0 ug/106 cells 1:4,000-1:8,000 1:1,000-1:4,000 1:5,000-1:10,000 |
| Application Info | Identification and enumeration of IgG1+ cells by immunofluorescence microscopy, Second step reagent for rat IgG1 monoclonal antibodies, ELISA, can be used as capture antibody |
| Other Applications Data | Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specificneeds. |
| Storage | - The purified antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH8.2. No preservatives or amine- containing buffer salts added; store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg and the Cyanine 5 (Cy5) conjugate is supplied as 0.1mg in1.0 mL of PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50%Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The HRP conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservativeadded. Store at 2- 8 C or long- term at - 20 C - The BIOT conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3; store at 2- 8 C - The R- PE conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent; store at 2- 8 C - FITC and R- PE conjugates should not be frozen and should be protected from prolonged exposure to light. Eachreagent is stable for the period shown on the bottle label if stored as directed. |
