Rat Anti-CD68 Monoclonal Antibody (clone: FA-11) APC conjugated
Katalog-Nummer CFB8823-25
Size : 25Assays
Marke : Cohesion Biosciences
Product Name: | Anti-CD68 Antibody-APC labled |
Cat No: | CFB8823 |
Source: | Rat |
Reactivity: | M |
Applications: | IF, FC |
*Application Key: | |
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish | |
*Species Reactivity Key: | |
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference |
Description:Rat monoclonal antibody APC labled to CD68Immunogen:Purified Con A receptor glycoproteins from the P815 cell linePurification:The antibody was purified by affinity chromatography.Clonality:Monoclonal (clone: FA-11)Conjugation:APCForm:Rat IgG2a. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.Dilution:10 μl / assayGene Symbol:CD68Alternative Names:Macrosialin; Gp110; CD68 Entrez Gene (Mouse): 12514; SwissProt (Mouse): P31996; Directions for Use:1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).Storage/Stability:Shipped and store at 4°C for one year. Do not freeze.