Key Features
- Rabbit Polyclonal
- Suitable for IHC on frozen and paraffin sections
- Reacts with human
Product not available in China
| Quantity | 50 µl (lyoph.) |
|---|---|
| Antibody Type | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Application | IHC |
| Purification | Undiluted antiserum |
| Reactivity | Human |
| Storage before reconstitution | 2-8°C until indicated expiry date |
| Storage after reconstitution | Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Extract from a substance P-producing ileal carcinoid |
| Formulation | Lyophilized; reconstitute in 100µl dist. water |
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:5-1:10 |
| Immunohistochemistry (IHC) - paraffin | 1:5-1:10 (microwave treatment recommended) |
EGC is a granule preparation from a substance P-producing carcinoid in the ileum. The EGC-antiserum recognizes most endocrine cells in the gut and the pancreas. The EGC-antiserum is a powerful tool in revealing the endocrine nature of tumors. Gastroentero-pancreatic endocrine tumors, medullary carcinoma of the thyroid and bronchial carcinoids are stained positive with the antiserum. Works on routinely processed specimens. Absorption with 10-100 ug EGC per ml diluted antiserum abolishes the staining.
Positive control: Bouin-fixed paraffin sections of human rectum.
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.